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PROFACTTMis a unique ready to use 3.2% buffered Tri-sodium citrate solution formulated for collection of blood for routine coagulation assays. The reagent contains 0.01% Thimerosal as preservative. Simple Steps for Making Sodium Citrate Buffer Nov 30, 2019 Sodium Citrate, 3.8% w/v Sodium Citrate, 3.8% w/v R014 Intended Use: Sodium citrate is used as anticoagulant to prevent blood from clotting. Composition** Ingredients Sodium citrate 3.80g Distilled water 100.0ml Final pH ( at 25°C) 7.9±0.1 **Formula adjusted, standardized to suit performance parameters Directions Dispense 0.5 ml of sodium citrate into test tube. Effects of anticoagulant on pH, ionized calcium

The 3.2% buffered sodium citrate binds less assay-added calcium than 3.8% buffered sodium citrate, therefore clotting times tend to be shorter in 3.2% than 3.8% buffered sodium citrate. 1 Although there are no exact data available, today 3.2% buffered citrate is rather widely used.

light blue-top vacuum tubes (3.2% buffered sodium citrate). Discard the pilot tube. Note: Reference intervals have been established using 3.2% buffered sodium citrate. 3. Fill light blue-top tubes as far as vacuum will allow and mix by gentle inversion five to six times. Exact ratio of nine parts blood to one part anticoagulant must be maintained. Buffered sodium citrate tubes - Kima BUFFERED SODIUM CITRATE TUBES The concentration of sodium citrate at 3,2% corresponds to a molarity 0,109. General Guidelines for Hemostasis and Thrombosis

Citrate tubes – plastic; Catalog no. Description Tube size Draw volume Color/ Closure Label Quantity (per box) Quantity (per case) e-catalog; 363083: Plastic citrate tube, .109 M/3.2%

Citrate Buffer (pH 3.0 to 6.2) preparation guide and recipe. Recipe can be automatically scaled by entering desired final volume. Citrate buffers can be used for RNA isolation, due to its ability to prevent base hydrolysis. The buffer is also used for antigen detection by breaking cross-links between antigens and any substances in its fixation 0.1M Citric acid-Sodium citrate buffer buffer – pH range 3.0 – 6.2 Prepare a 0.1M solution of citric acid monohydrate, C 6H8 O 7&H 2O (21.01g/l) and a 0.1M solution of trisodium citrate dihydrate, C 6H5 O 7Na 3&2H 2O (29.41g/l). Mix the volumes shown in the table. Or dissolve the masses shown and make up to 100cm 3 withwater calcium than 3.8% buffered sodium citrate, therefore clotting times tend to be shorter in 3.2% than 3.8% buffered sodium citrate.1 Although there are no exact data available, today 3.2% buffered citrate is rather widely used. A survey conducted in 2001 revealed that 156 of 593 US hospitals (26%) used the 3.8% buffered citrate for prothrombin time